By Aharon Oren (auth.), Russell H. Vreeland (eds.)
This e-book is designed to be an extended time period profession reference. The chapters current glossy techniques. it is a how-to-book with a distinction. those chapters:
- exhibit the history information regarding operating with salt loving organisms,
- are loaded with information regarding how experiments are performed below excessive salt,
- supply information regarding analyses that paintings lower than those stipulations and those who would possibly not,
- current a variety of info from laboratory designs to gear used or even to uncomplicated anecdotal tricks which can purely come from event.
Microbiological education focuses principally at the development, the dealing with and the research of the microbes linked to people and animals. but the biggest share of the Earth’s microbiota lives in saline environments equivalent to the Oceans, saline deserts and terminal hypersaline environments. This desire for salt will be intimidating for these attracted to coming into the sector or for these drawn to knowing how such learn is accomplished.
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Additional info for Advances in Understanding the Biology of Halophilic Microorganisms
Wais (1988) was successful in isolating haloarchaea using natural brines from thalassohaline lagoons and suggested that conventional media may be less effective for enrichment cultures. The early work of Schoop (1935) replaced NaCl in growth media with KCl, KNO 3 , Na2 CO3 , and NaNO3 . None of these replacements were suitable for obligate halophiles, but facultative halophiles grew with these substitutions. Based on the medium of Sehgal and Gibbons (1960; supplemented with FeCl2 ), studies on the extreme halophile Halobacterium cutirubrum have examined the effects of replacing NaCl with other salts by observing cell morphology and leakage (Abram and Gibbons 1961; Boring et al.
Appl Environ Microbiol 71:7352–7365 Stan-Lotter H, Leuko S, Legat A, Fendrihan S (2006) The assessment of the viability of halophilic microorganisms in natural communities. In: Rainey FA, Oren A (eds), Extremophiles—Methods in microbiology, vol. 35. Elsevier/Academic Press, Amsterdam Stephens DW, Gillespie DM (1976) Phytoplankton production in the Great Salt Lake, Utah, and a laboratory study of algal response to enrichment. Limnol Oceanogr 21:74–87 Stoeckenius W, Bivin D, McGinnis K (1985) Photoactive pigments in halobacteria from the Gavish sabkha.
Halotolerant bacteria typically have wider metabolic abilities than haloarchaea, thus, many carbon sources are suitable (Ventosa et al. 1982; Quesada et al. 1983; Litzner et al. 2006). Yeast extract is the most popular carbon source in complex media for halophilic and halotolerant bacteria and archaea (Dundas et al. 1963; Tomlinson and Hochstein 1972a,b; Post 1977; Rodriguez-Valera et al. 1980; Ventosa et al. 1982; Caton et al. 2004). It was a component of several Gibbons’ media (Abram and Gibbons 1960; Sehgal and Gibbons 1960) that form the bases of modern media recipes.