Animal Cell Culture Techniques by Shirley McBride, Mary Heenan, Martin Clynes (auth.), Dr.

By Shirley McBride, Mary Heenan, Martin Clynes (auth.), Dr. Martin Clynes (eds.)

Cell tradition concepts enable numerous molecular and telephone organic inquiries to be addressed, supplying physiological stipulations while keeping off using laboratory animals. as well as easy thoughts, a variety of specialized functional protocols overlaying the next parts are incorporated: phone proliferation and demise, in-vitro versions for cellphone differentiation, in-vitro types for toxicology and pharmacology, commercial program of animal telephone tradition, genetic manipulation and research of human and animal cells in culture.

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Add 90 ~l serum-free medium to each well of a V-bottomed cell culture microtiter plate. 6. Add 10 ~l of the first specimen to row A wells 1-6. 7. Similarly add 10 ~l of the second, third and fourth specimens to row A wells 7 -12, row E wells 1-6, and wells 7 -12, respectively. 8. Make seriall0-fold dilutions of each specimen from row A to row D and from row E to row H using 10 ~l volumes and a multichannel pipette. Fig. 4. Microtiter template 2 A B C D E F G H 3 4 5 6 7 8 9 10 11 12 2 Cell Culture and Diagnostic Virology 25 9.

2 Direct Culture Procedure This direct method of mycoplasma detection involves growing the mycoplasma on a selective agar and observing the colonies by microscopical examination. Mycoplasma can reproduce in a cell-free medium where, on agar, they exhibit a characteristic colony morphology, with the center of the colony embedded beneath the surface. Mycoplasma colonies in agar are viewed by microscope. m in diameter) with a dense center and a less dense periphery, giving the appearance of a fried egg (Fig.

5. Add 90 ~l serum-free medium to each well of a V-bottomed cell culture microtiter plate. 6. Add 10 ~l of the first specimen to row A wells 1-6. 7. Similarly add 10 ~l of the second, third and fourth specimens to row A wells 7 -12, row E wells 1-6, and wells 7 -12, respectively. 8. Make seriall0-fold dilutions of each specimen from row A to row D and from row E to row H using 10 ~l volumes and a multichannel pipette. Fig. 4. Microtiter template 2 A B C D E F G H 3 4 5 6 7 8 9 10 11 12 2 Cell Culture and Diagnostic Virology 25 9.

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