By Colin R. Howard, Howard
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Time is frequently of essence and Wulff and Lange (1975) reported that a positive diagnosis may be made by this technique within 72 h of receiving specimens in the laboratory. , 1984). , 1985). In general, electron microscopy is of use only in retrospective examination of confirmed positive fluids or biopsy material. , 1975). Both whole virions and viral antigens are more frequently seen in cells with a normal or near-normal ultrastructure, yet virus is rarely observed in areas of extensive hepa tocellular necrosis.
In the latter case, possible prior infection with LCM virus may account for simultaneous antibody to the second virus, particularly as Junin virus was successfully isolated in the acute phase of haemorrhagic fever in two patients, despite the concomitant presence of neutralizing antibodies to LCM virus. However, it is likely that patients showing exclusive seroconversion to LCM virus were in fact probably infected with LCM virus although clinically diagnosed as cases of Argentinian haemorrhagic fever.
Interestingly, the annual infection rate in both Oryzomys and Neacomys spp. appear to cycle (Fig. , 1977). , 1977). Originally known by its laboratory code (BeAn 293022), this agent is distinct from Amapari and is known as Flexal after a local tributary. This may also represent a single host-parasite relationship, there being no evidence of this virus in other rodent species trapped in the same area. Similarly, Pichinde virus was isolated from Oryzomys albigularis, a rodent which favours the broader regions of the riverine valleys draining the central uplands of Columbia (Trapido and Sanmartin, 1971).