By Sidney Fleischer, Lester Packer (Eds.)
The significantly acclaimed laboratory usual, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. for the reason that 1955, every one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this day - really an important ebook for researchers in all fields of existence sciences
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Additional info for Biomembranes Part G: Bioenergetics: Biogenesis of Mitochondria, Organization, and Transport
This is often the medium of choice, particularly for growing cells in the presence of radioactive tracers. Growth of cells (generation time about 150 min) is not as rapid in the YPD medium, but the low concentration of galactose minimizes catabolite repression of mitochondrial formation. Yields: 5--6 g cells per liter. 3. Synthetic medium: Some tracer studies may require a completely synthetic medium. 3% galactose. Growth on this medium is slow, however (generation time in excess of 250-350 min), and mitochondria are usually not fully developed.
Vesco, Nature (London), N e w Biol. 229, 136 (1971). 1~ A. M. Kroon and H. de Vries, in "Autonomy and Biogenesis of Mitochondria and Chloroplasts" (N. ), p. 318. , Amsterdam, 1971. ~ A. H. Scragg, Biochem. Biophys. Res. Commun. 45, 701 (1971). 13 E. A. Zylber, S. Perlman, and S. Penman, Biochim. Biophys. Acta 240, 588 (1971). 14 G. C. Godman, T. P. Keneklis, and M. E. King, Exp. Cell Res. 77, 159 (1973). ~5 C. Saccone and E. Quagliariello, Int. Rev. Cytol. 43, 125 (1975). ~e H. T. Abelson and S.
Chem. 37, 854 (1965). ] Protein Synthesis on Isolated Ribosomes Furthermore information about the mechanism of protein synthesis in both yeast and mammalian mitochondria can be obtained using isolated ribosomes in a "cell-free" system. For example, the sensitivity of protein synthesis to various antibiotics has been established using ribosomes obtained from yeast and liver mitochondria. 5, 10 mM MgC12, 10 mM Yeast cells are converted to spheroplasts and mitochondria are prepared in medium B as described above.