By Frédéric De Ceuninck (Editor), Massimo Sabatini (Editor), Philippe Pastoureau (Editor)
An fundamental selection of up-to-date classical and rising recommendations that promise so as to add serious wisdom to our figuring out of cartilage metabolism in future health and ailment. quantity 1: mobile and Molecular instruments describes confirmed molecular and mobile recommendations for the in vitro learn of standard and osteoarthritic cartilage via biochemical, biomolecular, immunological, and actual techniques, with emphasis at the genetic manipulation of cells. quantity 2: constitution and In Vivo research, bargains state of the art techniques for the study-at the tissue level-of turnover, constitution, and functioning of ordinary and diseased articular cartilage via invasive and noninvasive capability. accomplished and up to date, the 2 volumes of Cartilage and Osteoarthritis supply researchers and bench scientists with easily reproducible protocols for brand spanking new experiments to understand-from the mobile to the animal level-the pathophysiology of cartilage and to find molecular pursuits for pharmacological intervention.
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Extra info for Cartilage and Osteoarthritis Vol 2 Structure and In Vivo Analysis (Methods in Molecular Medicine)
1995) Discoordinate 15 gene expression of aggrecan and type II collagen in experimental osteoarthritis. Arthritis Rheum. 38, 420–425. 16. , and von der mark, K. (1997) Activation of fibrillar collagen synthesis and phenotypic modulation of chondrocytes in early human osteoarthritis cartilage lesions. Osteoarthritis Cartilage 5, 183–189. 17. , et al. (2002) Processing of type II procollagen 17 aminopropeptide by matrix metalloproteinases. J. Biol. Chem. 277, 2193–2201. 18. , Chapuy, M. , and Delmas P.
Osteoarthritis patients. 6 yr). They consulted at the Department of Rheumatology of the Centre Hospitalier Lyon Sud (Lyon, France). All patients fulfilled the American College of Rheumatology criteria for primary knee OA (20). All patients had had chronic daily pain of the knee for at least 3 mo (median: 10 yr) and also had radiographical evidence of OA with joint space narrowing when using the posteroanterior view of the knees flexed at 30° (Schuss view). Patients presenting with an advanced stage of OA with a minimum joint space width of less than 1 mm were excluded.
1. Safety Considerations 1. The microtome and knife should be treated with respect; these are precisely engineered instruments manufactured to very fine tolerances. 2. Caution: Many of the chemicals associated with histochemical processing are hazardous to health. Pregnant staff should take particular care, as many of the chemicals routinely used in histochemical processing can cause fetal damage or death. 3. Tissue processors should be kept in rooms with efficient fume extraction. 4. All transfers of chemicals from storage bottles to tissue processor containers should be performed in a spark-proof fume hood.