By Victor Ginsburg (Eds.)
The severely acclaimed laboratory ordinary, Methods in Enzymology, is likely one of the so much hugely revered courses within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - actually an important booklet for researchers in all fields of lifestyles sciences
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Additional resources for Complex Carbohydrates Part E
Andrews, P. N. Goodfellow, L. H. Shevinsky, D. L. Bronson, and B. B. Knowles, lnt. J. Cancer 29, 523 (1982). 34 E. G. Bremer, S. B. Levery, S. Sonnino, R. Ghidoni, S. Canevari, R. Kannagi, and S. Hakomori, J. Biol. Chem. 259, 14773 (1984).  MICROSCALE METHYLATION ANALYSIS OF GLYCOLIPIDS m/z 292 23 m/z 348 1 41437 ~ql 42 • I4 264 ,4 oz 6 AI 5~ 520 6e 6, 715 262 ~()I 292 1852 I] 234 264 FUG. 4. GC-MS on DB-225 of PMAAs from Fncal --, 2Galfll --~ 3Gb4Cer sample used in Fig. 3. Peaks are identified with the same numbers as in Fig.
1). , Rockford, IL) conveniently fit into these conical tubes. , acetic anhydride (Baker, reagent grade, distilled over sodium acetate before use), "ultrapure" sulfuric acid and acetic acid (Alfa Products, Danvers, MA), chloroform (Baker, nanograde), dimethyl sulfoxide (Pierce, silylation grade), hexane and methanol (Baker, HPLC grade), methyl iodide (Baker, reagent grade, distilled before use), sodium tetradeuteridoborate, NaBD4 (Alfa Products), sodium hydride (Alfa Products, 80% dispersion in oil, washed with hexane before use), and sodium hydroxide (Baker "Dilut-it," free of carbonate).
Et20 as the catalyst, for example, the glycosidic bond between 6linked glycopyranosyl residues is relatively stable to reductive cleavage, and preliminary results indicate that fl-1,3- and r-1,4-1inked glucopyranosyl residues are not cleaved at all. This selectivity is likely to be very useful in sequence studies. Experimental protocols for carrying out this reaction and the results obtained with some model permethylated polysaccharides are given in the following sections. Experimental Procedures Reductive Cleavage with Et3SiH and TMSOTf.