Culture of Epithelial Cells, Second Edition by R. Ian Freshney(eds.)

By R. Ian Freshney(eds.)

"...a brilliant compendium of present in vitro techniques that would be an invaluable source to these simply beginning to paintings with an epithelial cellphone method in addition to those who were operating with them for years and years."
Pharmaceutical Research

This thoroughly revised and multiplied new version offers designated descriptions of primary and useful elements in relation to the in vitro cultivation of disparate kinds of epithelia. lately, using epithelial phone tradition in mobilephone biology and tissue engineering has elevated dramatically. This revision displays these advances through together with new chapters at the tradition of animal and human hepatocytes, kidney epithelium, and bladder epithelium. each one bankruptcy presents an introductory assessment of the rules and merits of the actual approach, through exact protocols, functional information, exchange equipment, and an invaluable checklist of fabrics and suppliers.Content:
Chapter 1 advent (pages 1–30): R. Ian Freshney
Chapter 2 cellphone interplay and Epithelial Differentiation (pages 31–63): Nicole Maas?Szabowski, Hans?Jurgen Stark and Norbert E. Fusenig
Chapter three The skin (pages 65–94): E. Kenneth Parkinson and W. Andrew Yeudall
Chapter four tradition of Human Mammary Epithelial Cells (pages 95–135): Martha R. Stampfer, Paul Yaswen and Joyce Taylor?Papadimitriou
Chapter five tradition of Human Cervical Epithelial Cells (pages 137–169): Margaret A. Stanley
Chapter 6 Human Prostatic Epithelial Cells (pages 171–194): Donna M. Peehl
Chapter 7 Human Oral Epithelium (pages 195–255): Roland C. Grafstrom
Chapter eight common Human Bronchial Epithelial telephone tradition (pages 257–276): John clever and John F. Lechner
Chapter nine Isolation and tradition of Pulmonary Alveolar Epithelial sort II Cells (pages 277–301): Leland G. Dobbs and Robert F. Gonzalez
Chapter 10 Isolation and tradition of Intestinal Epithelial Cells (pages 303–335): Catherine sales space and Julie A. O'Shea
Chapter eleven Isolation and tradition of Animal and Human Hepatocytes (pages 337–379): Christiane Guguen?Guillouzo
Chapter 12 tradition of Human Urothelium (pages 381–399): Jennifer Southgate, John R.W. Masters and Ludwik ok. Trejdosiewicz
Chapter thirteen different Epithelial Cells (pages 401–436): R. Ian Freshney

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Example text

7. Cross-Contamination. . . . . . . . . . . . . . . . . 8. Differentiation . . . . . . . . . . . . . . . . . . . . 1. Induction of Differentiation . . . . . . . . . . . 9. Transformation . . . . . . . . . . . . . . . . . . . . References. . . . . . . . . . . . . . . . . . . . . . . . Appendix: Sources of Materials. . . . . . . . . . . . . . . 2 3 3 5 5 5 6 6 7 7 7 8 9 10 12 13 20 24 30 ‘‘Epithelium’’ describes the various layers of cells that either coat surfaces on the exterior of the organism or line internal Culture of Epithelial Cells, pages 1–30 organs, ducts, or secretory acini.

1988 Introduction 21 control, which produces a more rapidly growing cell that is no longer anchorage-dependent. , 1991]. , anchorage-independent growth, loss of contact inhibition of cell migration, and loss of density limitation of cell proliferation or topoinhibition). , in contact-inhibited 3T3 cell lines), it is better to use the term immortalization and reserve in vitro transformation for cells that show altered growth control. Usually, but not always, the development of autonomous growth control (lower serum growth factor dependence), loss of contact inhibition and topoinhibition, and anchorage independence correlate with malignancy and they may be the same as, or at least a major component of, malignant transformation.

7. Cross-Contamination. . . . . . . . . . . . . . . . . 8. Differentiation . . . . . . . . . . . . . . . . . . . . 1. Induction of Differentiation . . . . . . . . . . . 9. Transformation . . . . . . . . . . . . . . . . . . . . References. . . . . . . . . . . . . . . . . . . . . . . . Appendix: Sources of Materials. . . . . . . . . . . . . . . 2 3 3 5 5 5 6 6 7 7 7 8 9 10 12 13 20 24 30 ‘‘Epithelium’’ describes the various layers of cells that either coat surfaces on the exterior of the organism or line internal Culture of Epithelial Cells, pages 1–30 organs, ducts, or secretory acini.

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