Culture of Marine Invertebrate Animals: Proceedings — 1st by John M. King (auth.), Walter L. Smith, Matoira H. Chanley

By John M. King (auth.), Walter L. Smith, Matoira H. Chanley (eds.)

This quantity relies on prec'entations on the convention on tradition of Marine Invertebrate Animals which was once held in eco-friendly­ port, manhattan in October, 1972. The convention used to be backed by means of the center Atlantic common Sciences Council, Inc., a non­ revenue academic company, including the Marine technological know-how facilities of Adelphi collage, the kingdom collage of latest York at Stony Brook, new york collage, Suffolk County group collage, and the defend Island Oyster corporation. the aim of the convention used to be to supply a wanted ex­ switch of information between scientists of assorted specialties whose details will be beneficial to others faced with comparable difficulties, regardless of assorted marine animals. half I considers supportive ideas -- common isolation and tradition tools, difficulties of affliction and feeding. particular strategies hired within the tradition of a variety of invertebrate organisms is roofed partly II. we wish to thank the members for his or her cooperation in getting ready the manuscripts in response to their convention shows. Walter L. Smith Matoira H. Chanley v Contents half I Recirculating method tradition equipment for Marine Organisms .............•.

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Example text

Fernbach cultures can be sampled (for counting) fairly easily through the inoculating port or by pulling the plug, but either process may be difficult for carboy cultures. In this case provide a glass siphon tube in the carboy, joined to latex or plastic tubing outside. This tubing is joined by a piece of 5 mm glass tube about 7 cm long to a #1 two-hole stopper; through the other hole of the stopper is passed another piece of 5 mm glass tube, bent at about right angles. This bent tube is stuffed with a piece of cotton (as is a pipette).

Larger plugs are made in much the same way. Plugs can accommodate aeration tubes, siphons, and even tubes to be used as inoculation ports, if desired. Use several layers of cheesecloth, and punch holes through it and the cotton swatch for the tubings Space the tubings as desired with cotton and tie with white string, then fold up the cheesecloth-covered swatch and tie. It is usual to put covers of aluminum foil over the stoppers of stock culture flasks as additional dust protection. Squares of foil are pressed gently down over the plugs then squeezed around the neck of the flask; the foil should be loose enough so that it comes off easily without pulling the plug when picked up by one corner.

This tubing is joined by a piece of 5 mm glass tube about 7 cm long to a #1 two-hole stopper; through the other hole of the stopper is passed another piece of 5 mm glass tube, bent at about right angles. This bent tube is stuffed with a piece of cotton (as is a pipette). The stopper is now inserted into a 16-18 mm x 150 mm test tube. In effect one of them has a two-stage siphon, with the test tube acting as a reservoir. Suction applied (by pump or mouth) to the bent glass tube will draw li~uid from the culture into the test tube; when suction is released the siphon is broken provided the culture tube is above the level of li~uid in the carboy.

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