By M. H. K. Shokeir, Y. Lefebvre (auth.), J. Bain, E. S. E. Hafez (eds.)
Despite the expanding variety of andrological pub opment of uncomplicated and medical study in andrology, lications, the diagnostic facets of andrology need to research sleek options for the evaluate of bought particularly little awareness. within the final decade male infertility, to stimulate the improvement of considerable development has been made within the less than guideliness for healing techniques, to recom status of the basics of andrology, this ment universal norms of dimension, to advertise development due to glossy thoughts and and interchange of data and to stimulate the instrumentation in microanatomy, immunology, curiosity of scientists and clinicians in andrological neurophysiology, pathology, genetics, endocrino difficulties. logy, biochemistry, biophysics, urology and sur we're thankful to the authors who've given so gery. those stories are scattered in any such large a lot in their time and abilities to provide chapters spectrum of journals that andrologists can hardly ever of intensity and breadth and who've made this kind of hold abreast of the advances. there were major contribution to the andrological litera textbooks at the testes, male accent organs and ture. we're additionally indebted to Morag M. Smith, Lori semen yet none that experience tried to deliver Rust and Penny Stoops for the time and persistence jointly a few of the facets of diagnosis.
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Additional info for Diagnosis in Andrology
The patients studied by Walsh et al. (1974) had normal circulating T and DHT; however, 5-ctreductase activity in slices of genital skin was deficient. On this basis, Walsh postulated that the male pseudohermophroditism in these patients was due to deficient 5-ct-reductase in androgen target tissue only. However, Imperato-McGinley et al. (1974) and Saenger et al. (1978) demonstrated low DHT concentration in sera of patients with 5-ctreductase deficiency. Whereas Pinsky et al. (1972) and Wilson (1975) reported that the 5-ct-reductase defect was demonstrable only in fibroblasts from genital skin, Prostate Semi na I Urethra Urogenital SinUS / -------~ Blind vagi nal pouch Figure 8.
18 17 'I E 21 22 )( G )( b Skin, including genital skin, offers an extensive and topologically diverse source of somatic cells with high division potential. The fibroblasts in the skin biopsy specimens tend to outgrow the epithelial cells. Their chromosomes can be best visualized and studied during the metaphase stage of the mitotic division of the cultured cells. This stage is usually attained in a few weeks of culture of the skin explant. Fragments from other tissues and viscera, if their cells possess or can be made to re-acquire the mitotic potential, may be likewise treated and investigated with a view to ascertaining the makeup of their chromosomes both in terms of number and individual structure.
The 'inducer substance' for testicular organogenesis proposed by Jost appears to be H-Y antigen. This antigen is the first plasma membrane or cell surface protein to which a specific organogenesis function has been assigned (Ohno, 1977) (Fig. 1). 2. Morphologic processes in primary sex determination Until the 12 mm stage (at approximately 42 days of gestation), the gonads of the male and female are morphologically indistinguishable. During the 7th week of fetal life the differentiation of the initially bipotential gonad begins.