Fertilization and Embryonic Development In Vitro by John D. Biggers (auth.), Luigi Mastroianni Jr., John D.

By John D. Biggers (auth.), Luigi Mastroianni Jr., John D. Biggers (eds.)

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Gynecol. 117:587. Edwards, R. , and Steptoe, P. , 1975, Induction of follicular growth, ovulation and luteinization in the human ovary, J. Reprod. Fertil. Suppl. 22: 121. Goodman, A. , Nixon, W. , Johnson, D. , and Hodgen, G. , 1977, Regulation of folliculogenesis in the cycling rhesus monkey: Selection of the dominant follicle, Endocrinology 100: 155. Hodgen, G. , Tullner, W. , Vaitukaitis, J. , Ward, D. , and Ross, G. , 1974, Specific radioimmunoassay of chorionic gonadotropin during implantation in rhesus monkeys, J.

More recently, we have found it necessary to measure serum LH more frequently, such as at 6-hr intervals. This markedly improved the predictability of follicular status. , 1971) without bovine serum albumin (BSA). In most instances, the antral cavity was gently flushed two to three times in order to maximize the rate of ovum recovery. The contents of the aspirate were examined under a dissecting microscope immediately thereafter. The oocyte, when present, was classified as apparently mature, intermediate, or immature on the basis of morphological criteria (Soupart and Morgenstern, 1973; Edwards and Steptoe, 1975; Kreitmann and Hodgen, 1980); that is, the surrounding corona-cumulus investment, the presence or absence of a germinal vesicle in the ooplasm, or the first polar body in the perivitelline space.

Steril. 34: I. Bennett, J. , 1967, The induction of ovulation in the squirrel monkey (Saimiri sciureus) with pregnant mares serum (PMS) and human chorionic gonadotropin (HCG), J. Reprod. Fertil. 13:357. Bennett, W. , Dufau, M. , Catt, K. , and Tullner, W. , 1973, Effect of human menopausal gonadotropin upon spermatogenesis and testosterone production in juvenile rhesus monkeys, Endocrinology 92:813. Biggers, J. , Whitten, W. , and Whittingham, D. , 1971, The culture of mouse embryos in vitro, in: Methods in Mammalian Embryology (J.

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