High Resolution Separation and Analysis of Biological by Abelson J.N., Hancock W.S., Simon M.I. (eds.)

By Abelson J.N., Hancock W.S., Simon M.I. (eds.)

The significantly acclaimed laboratory typical for greater than 40 years, equipment in Enzymology is without doubt one of the such a lot hugely revered courses within the box of biochemistry. on account that 1955, each one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. greater than 260 volumes were released (all of them nonetheless in print) and lots more and plenty of the fabric is proper even this present day - really a necessary booklet for researchers in all fields of lifestyles sciences.Key positive factors* Liquid chromatography* Electrophoresis* Mass spectrometry

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Additional info for High Resolution Separation and Analysis of Biological Macromolecules: Fundamentals Part A

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L. Karger,J. Chrornatogr. 371, 3 (1986). 36 LIQUID CHROMATOGRAPHY [2] TABLE I C()MMER('IAI ANALYI ICAL HIC COI,UMNS Manufacturer (location)/name J. T. Baker (Phillipsburg, NJ) BakerBond HI-propyl BakerBond PREPSCALE Beckman (Fullerton, CA) Spherogel CAA-HIC5 Pharmacia (Piscataway, NJ) Phenyl-Superose Alkyl-Superose PerSeptive Biosystems (Cambridge, MA) PH PE BU ET PolyLC (Columbia. 5/xm/100 A. , 500 ,~, 1000 30/xm/300 A Polyamide coating with ligand indicated/silica 10/~m/1000 10 ~m/1000 A. 5 /xm, nonporous Phenyl/polymer Oligopolycthylene glycol/polymer n-Butyl/polymer to high-speed protein separations.

J. Hiyama, A. Surus, and A. G. C. 1. Endocrinol. 12S, 493 (1990). ,~4 M. A. Chlenov, E. I. Kandyba, L, V. Nagornaya. I. L. Orlova, and Y. V. Volgin, J. C7tromatogr. 631, 261 (1993). ~,5S. C. G o h e c n and S. C. Engelhorn. J. Chromatogr. 326, 235 (1985). ~'e'S. A. Berkowitz and M. P. Henry, J. Chromatogr. 389, 317 (1987). ,7 E. Harlow and D. " Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1988. Fl~;. 11. 46 x: 15 cm (C) columns. M e t - h G H and r h G H were injected separately and overlaid together in the same chromatogram.

Column type, mobile-phase pH, temperature, and additives). For example, in Fig. 3, methionyl human growth hormone (in which an extra methionine residue is on the N-terminal sequence of native growth hormone) can be better separated from native human growth hormone by using a more hydrophobic phenyl stationary phase than an ether column) s In this study, the temperature and pH can also affect the separation. 3s It should be pointed out 33 y. Kato, T. Kitamura, K. Nakamura, A. Mitsui, Y. Yamasaki, and T.

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