Human Retrovirus Protocols: Virology and Molecular Biology by Tuofu Zhu

By Tuofu Zhu

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Extra resources for Human Retrovirus Protocols: Virology and Molecular Biology (Methods in Molecular Biology 304)

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13. On d 7, cells can be used for HIV-1 infection. 2. Monocyte Propagation 1. Suspend monocytes in monocyte medium with MCSF at 1 × 106 cells/mL. Gently mix the cells by pipetting to obtain uniform suspension. Plate the cells immediately after elutriation. 2. Plate an appropriate volume of cells for type of plate or culture dish (see Table 1). 3. Incubate at 37°C with 5% CO2 and 95% humidity. 4. On d 3, add additional monocyte medium with MCSF (as shown in Table 1) and incubate under the same conditions.

Zack, J. , Birch, C. , et al. (2001) Macrophage tropism of human immunodeficiency virus type 1 isolates Isolation of HIV-1 From Monocytes 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 33 from brain and lymphoid tissues predicts neurotropism independent of coreceptor specificity. J. Virol. 75, 10,073–10,089. , Mutimer, H. , et al. (2001) Monocytes harbour replication-competent, non-latent HIV-1 in patients on highly active antiretroviral therapy. AIDS 15, 17–22. Crowe, S. M. and Sonza, S. (2000) HIV-1 can be recovered from a variety of cells including peripheral blood monocytes of patients receiving highly active antiretroviral therapy: a further obstacle to eradication.

1997) Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278, 1295–1300. Chun, T. , Mizell, S. , Ehler, L. , Mican, J. , et al. (1997) Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Proc. Natl. Acad. Sci. USA 94, 13,193–13,197. Wong, J. , Gunthard, H. , Havlir, D. , Ignacio, C. , Spina, C. , and Richman, D. D. (1997) Recovery of replication-competent HIV despite prolonged suppression of plasma viremia.

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