Imaging in cellular and tissue engineering by Hanry Yu, Nur Aida Abdul Rahim

By Hanry Yu, Nur Aida Abdul Rahim

"This booklet covers the total diversity of obtainable imaging modalities and optical equipment used to aid overview fabric and organic habit. It additionally highlights quite a lot of optical and organic purposes. every one bankruptcy within the textual content describes a particular program and discusses correct instrumentation, governing actual ideas, facts processing strategies, in addition to merits and drawbacks of each  Read more...

OVERVIEW creation to mobile and Tissue Engineering (CTE), Hanry Yu IN VITRO purposes Confocal Microscopy for mobile Imaging: High-Content research, Barry Masters Two-Photon Microscopy for Scaffold Characterization, Chen-Yan Dong Two-Photon Microscopy for floor Mapping and Organ Characterization, Dean Tai AFM for mobilephone and Tissue area of interest, solar Wanxin CT/MRI for Computer-Aided Tissue Engineering, Wei sunlight quickly Micro-CT for In Vitro program, notebook Cheng IN VIVO purposes X-Ray and Scintigraphy functions for Tissue Engineering, Benjamin Harrison Ultrasound Imaging in Gene and Drug supply, Katherine Ferrara MRI to watch Implanted Constructs, Athanassios Sambanis PET/SPECT to observe Implanted Constructs, David Townsend CT/Micro-CT to watch Implanted Constructs, Teoh Swee Hin Two-Photon Imaging of Bone Marrow, Charles Lin Intravascular OCT, Linbo Liu Confocal Imaging for Ocular floor irritation, Roger Beuerman Small Animal Optical Imaging, Robert Hoffman entire Animal Imaging in Gene supply, Wang Shu Imaging in Metabolic medication, Weiping Han facts research photograph Processing for mobile Engineering, Felix Margadant photo research for mobile Engineering, Jagath Rajapakse Confocal Microscopy for big inhabitants Imaging, James Evans viewpoint at the way forward for CTE, Hanry Yu

Table of Contents

Overview
Introduction to mobile and Tissue Engineering, Hanry Yu and Abhishek Ananthanarayanan

In Vitro Applications
Confocal Microscopy for mobile Imaging: High-Content Screening, Baixue Zheng and Abhishek Ananthanarayanan
Use of Multiphoton Microscopy for Tissue Engineering functions, Wei-Liang Chen, Hsuan-Shu Lee, and Chen-Yuan Dong
Two-Photon Microscopy for floor Mapping and Organ Characterization, Anju Mythreyi Raja
Atomic strength Microscopy for phone and Tissue area of interest, Wanxin Sun

In Vivo Applications
Magnetic Resonance Imaging to observe Implanted Constructs, Nicholas E. Simpson and Athanassios Sambanis
Application of Imaging applied sciences to Stem-Cell monitoring In Vivo, Sheng-Xiang Xie and Kishore Kumar Bhakoo
Computer Tomography and Micro-CT for Tissue Engineering program, Zhang Zhiyong, Jerry K.Y. Chan, and Teoh Swee Hin
Intravascular Optical Coherence Tomography, Liu Linbo and Atsushi Tanaka
Imaging of healing techniques in Animals utilizing Optical Reporter Genes, Ying Zhao, Jiakai Lin, and Shu Wang
Imaging in Metabolic medication, Jinling Lu, Kishore Kumar Bhakoo, Kai-Hsiang Chuang, George okay. Radda, Philip W. Kuchel, and Weiping Han
High-Resolution X-Ray Cone-Beam Microtomography, Xiaochun Xu and Ping-Chin Cheng

Data Analysis
Image research for mobile and Tissue Engineering, Shuoyu Xu, Piyushkumar A. Mundra, Huipeng Li, Shiwen Zhu, Roy E. Welsch, and Jagath C. Rajapakse

Index

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Wong, M. L. et al. 1998. Polymerization of purified yeast septins: Evidence that organized filament arrays may not be required for septin function. J Cell Biol, 143, 737–749. Friedman, S. L. 2008. Mechanisms of hepatic fibrogenesis. Gastroenterology, 134, 1655–69. Garner, J. P. 2004. Tissue engineering in surgery. Surgeon, 2, 70–8. Gauthier, N. , Fardin, M. , and Sheetz, M. P. 2011. Temporary increase in plasma membrane tension coordinates the activation of exocytosis and contraction during cell spreading.

Once the nuclei are correctly segmented, it can be used to guide the segmentation of cell cytoplasm. The simple approach is based on the assumption that cell edge is at roughly equal distance to their respective nuclei. The algorithm enlarges the nuclear channel mask until the neighboring touches each other and the touching edge is defined to be the cell edge. , 2010). 5 Example of Cellular Features from an HCS Study Size Shape Fluorescence Intensity Total surface area Circularity Total intensity Mean surface area Geometry centroid Average intensity Median surface area Variance of surface area Center of mass Perimeter Length of major axis Length of minor axis Mean intensity Median intensity Variance of intensity Distribution Mean distance to other objects Median distance to other objects demanding and slower, however, it generally generates better results for cells with extended morphology such as neuronal cells.

3 Image Processing and Statistical Analysis Software Image-processing software serves to remove background noise, segment cells, and convert image into numerical values for quantitative analysis. Image-processing protocols should be robust enough to handle images with variable quality. The image quality can vary drastically owing to a variety of reasons such as imperfect focus depth, impurity in the sample, bubbles, and uneven cell density. In addition, segmentation algorithm should be optimized according to the samples as factors such as cell shape, intensity distribution, and cell density can affect the performance of the segmentation algorithm.

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