Invertebrate Cell Culture Applications by Karl Maramorosch

By Karl Maramorosch

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1a, 1 d ) . On the other hand, the cephalic complexes of the Bar mutant promoted the Bar type morphogenesis for both wild-type and Bar eye-antennal discs (Fig. 1b, 1 e ) . Many other morphological mutants of the eye in D. melanogaster have also been tested for the activity of the cephalic complexes to promote the differentiation of eye-antennal discs and for the responsiveness of the eye-antennal discs to cephalic complexes (Horikawa, 1960; F u j i o , 1 9 6 0 ) . 2) mutant had a similar effect as the Bar cephalic complex in promoting the differentiation of the eye-antennal discs.

Tyrosine decreased to some extent in most of the cultures. However, it increased in the early stage and then decreased in the later stages of the Carpocapsa pomonella cell culture (Fig. 2 1 ) . From the analyses of free amino acids in media, it became evident that each cell line has characteristic patterns of amino acid metabolism. The Papilio xuthus cell line is characterized by production of glutamic acid; the Mamestra brassicae ovary cell line by production of ß-alanine and proline and marked consumption of histidine and tryptophan; the Mamestra brassicae fat body cell line by marked consumption of 3-alanine, cystine, glutamine, isoleucine, leucine, tyrosine, and valine; the Aedes albopictus cell line by greater consumption of aspartic acid, glutamic acid, slightly increased consumption of glycine and arginine, and production of proline; the Aedes aegypti cell line by production of ß-alanine and histidine; the Culex molestus cell line by production of glutamic acid; and the Antheraea eucalypti cell line by production of glycine and marked consumption of asparagine.

8, 329-332. Patterson, Μ. , Jr. (1972). In "Growth, Nutrition, and Metabolism of Cells in Cultures" (G. Η. Rothblat and V. J. Cristofalo, e d s . ) , V o l . 1, pp. 171-209. Academic Press, New York. Rehàcek, J. and Brzostowski, H. W. (1969). J. Insect Physiol. 15, 1431-1436. Singh, P. (1977). "Artificial Diets for Insects, M i t e s , and Spiders," pp. 1-594. IFI/Plenum, New York. Wyatt, S. S. (1956). J. Gen. Physiol. 39, 841-852. Wyatt, G. , Loughheed, T. , and Wyatt, S. S. (1956). J. Gen. Physiol.

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