Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea: by M. S. McNulty, W. L. Curran, J. B. McFerran (auth.), P. W.

By M. S. McNulty, W. L. Curran, J. B. McFerran (auth.), P. W. de Leeuw, P. A. M. Guinée (eds.)

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Extra resources for Laboratory Diagnosis in Neonatal Calf and Pig Diarrhoea: Proceedings of a Workshop on Diagnostic Techniques for Enteropathogenic Agents Associated with Neonatal Diarrhoea in Calves and Pigs, held at the Central Veterinary Institute, Department of Virology

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Specificity and sensitivity of this type of assay are highly dependent on the availability of high titered antisera possessing high specificity. Usually such sera are prepared by hyperimmunization of rabbits, guinea pigs or goats with purified antigen. Prerequisites are that the animals have very low pre-immunization antibody levels, that the antigen preparation used for hyperimmunization is free of contaminating proteins and that the appropriate antigenic determinants are present. , 1977). Similar problems are well-known in the purification of coronaviruses.

In contrast, the virus was found in 7 specimens only out of 97 samples collecteddayly from 10 healthy calves and in one calf only out of 19 healthy calves. In the same herds a total of 60 faecal samples, collected from dams at the time of parturition and a total of 20 other specimens from cows were analyzed for the presence of rotavirus. As shown in table II, all these specimens were negative. 44 3. ELISA for the detection of rotavirus immune complexes. The technique (see materials and methods) was applied to the detection of immune complexes in the faeces of pigs.

1978). The ELISA-inhibition technique, as shown in Fig. ld, may also be listed under this heading. The test measures the ability of putative antigen-containing specimens to inhibit the binding of a limited amount of antiviral antibody to bound viral antigen. This assay does not require the use of high titered antiviral antisera in contrast with all other enzyme immunoassays for the detection of antigens. , 1980). Trapping system. The most widely used method for the detection of antibody is the indirect enzyme immunoassay (Fig.

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