Laboratory Procedures and their Applications by Indra Vasil

By Indra Vasil

Laboratory methods and Their functions

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Laboratory Procedures and their Applications

Laboratory techniques and Their purposes

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Initiatio nfro mSeed s Seedlings are grown on numerous substrates in different types of vessels in the greenhouse or growth chambers. Preference is given to axenic conditions which are indispensable for excised embryos. Usually, seeds are easily decontaminated by procedures described in Chapters 11 and 13, this volume. In some orders, achenes or caryopses are germinated. Success in sterilization of these fruits is usually rather low. The best results are obtained when they are used as long as the pericarps are alive.

Similar procedures can be used for other cytokinins. 4 2 4 2 2 4 2 4 2 2 2 2 2 3. Plant Cell Cultures: Nutrition and Media 25 2. Preparation of B5 medium. 0 ml 3. MS medium (for composition, see Table I). a. MS-micronutrient stock solution (keep frozen). 5 b. All other stock solutions are prepared the same as for B5. 4. Preparation of MS medium. 0 ml REFERENCE S Adams, T. , and Townsend, J. A. (1983). A new procedure for increasing efficiency of protoplast plating and clone selection. Plant Cell Rep.

1983). Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117, 4 0 - 4 4 . Rangan, T. S. (1974). Morphogenic investigations on tissue cultures of Panicum miliaceum. Z. Pflanzenphysiol. 72, 4 5 6 - 4 5 9 . Rangan, T. , and Vasil, I. K. (1983). Somatic embryogenesis and plant regeneration in tissue cultures of Panicum miliaceum L. and Panicum miliare Lamk. Z. Pflanzenphysiol. 109, 4 9 - 5 3 . , and Yamada, Y. (1979). Wheat plants regenerated from embryo cell cultures.

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