Methods in Protein Design by Amy E. Keating (Eds.)

By Amy E. Keating (Eds.)

This new quantity of Methods in Enzymology maintains the legacy of this foremost serial by means of containing caliber chapters authored through leaders within the box. This quantity covers equipment in protein layout and it has chapters on such issues as protein change engineering through area insertion, evolution established layout of proteins, and computationally designed proteins.

  • Continues the legacy of this optimum serial with caliber chapters authored through leaders within the field
  • Covers equipment in protein design
  • Contains chapters with such themes as protein swap engineering through area insertion, evolution-based layout of proteins, and computationally designed proteins

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RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways Edited by LYNNE E. MAQUAT AND MEGERDITCH KILEDJIAN VOLUME 450. Fluorescence Spectroscopy Edited by LUDWIG BRAND AND MICHAEL L. JOHNSON VOLUME 451. Autophagy: Lower Eukaryotes and Non-Mammalian Systems (Part A) Edited by DANIEL J. KLIONSKY VOLUME 452. Autophagy in Mammalian Systems (Part B) Edited by DANIEL J. KLIONSKY VOLUME 453. Autophagy in Disease and Clinical Applications (Part C) Edited by DANIEL J. KLIONSKY VOLUME 454.

C) Affinity maturation using yeast cell-surface display can further validate the accuracy of the design. Identification of mutations conferring affinity increases at the designed surface (left panel) suggests precision of the design if the improvements can be rationalized posteriori. Conversely, mutations conferring affinity increases distal to the designed surface (right panel) provide important clues that the protein is not binding the target as designed. than the starting design helps, by retroactive rationalization, to improve the design process and identify inaccuracies in the energy function (Fig.

While every screening method to identify 10 Timothy A. Whitehead et al. putative binders has its advantages and pitfalls, the ideal features of a screening method would be the following: that the throughput of the experimental screening method be matched to that of the computational design process, that diverse proteins can be robustly expressed, that weak binders (dissociation constants in the micromolar range; designs with fast kinetic dissociation rates) be positively identified, and that false negatives and false positives be minimized.

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