Methods of Biochemical Analysis, Volume 17 by David Glick

By David Glick

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22. B. Chance and B. Heaa, Science, 189, 700 (1959). 23. L. C. Clark, Trans. Am. Soc. Artificial Internal Organs, 3,41 (1956). 24. M. A. Lessler, E. Molloy, and C. M. , $4, 336 (1965). 25. A. Michaels, J. , 60, 393 (1961). 26. R. M. Hill and I. , J. Am. , 36,873 (1964). 27. R. M. Hill and I. Fatt, Nature, $00,1011 (1963). 28. F. G. Carey and J. M. Teal, J. Appl. , 20, 1074 (1965). 29. U. Gleichmann and D. W. Lubbers, Arch. Qes. , 871, 431 (1960). 30. I. M. Kolthoff and J. J. Lingane, Polarography, Interscience, New York, 1952.

19, 550 (1964). 22. B. Chance and B. Heaa, Science, 189, 700 (1959). 23. L. C. Clark, Trans. Am. Soc. Artificial Internal Organs, 3,41 (1956). 24. M. A. Lessler, E. Molloy, and C. M. , $4, 336 (1965). 25. A. Michaels, J. , 60, 393 (1961). 26. R. M. Hill and I. , J. Am. , 36,873 (1964). 27. R. M. Hill and I. Fatt, Nature, $00,1011 (1963). 28. F. G. Carey and J. M. Teal, J. Appl. , 20, 1074 (1965). 29. U. Gleichmann and D. W. Lubbers, Arch. Qes. , 871, 431 (1960). 30. I. M. Kolthoff and J. J. Lingane, Polarography, Interscience, New York, 1952.

Chromatography of this preparation by gel filtration on Sephadex LH-20 in 50% aqueous ethanol shows the presence of several pigments, with bilirubin diglucuronide as the main component (13). The solid substance contains 70-90010 bilirubin conjugates, determined as the diglucuronide. A preparation containing bilirubin diglucuronide as the only colored substance and with purity varying from 30 to 60% is obtained by the above-mentioned chromatography followed by evaporation at low temperature. The amorphous substance may be kept for several days a t -20" (13).

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