Molecular Biology of Iridoviruses by Dawn B. Willis (auth.), Gholamreza Darai (eds.)

By Dawn B. Willis (auth.), Gholamreza Darai (eds.)

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15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. Xeros, N. Nature 174: 562-563, 1954. Smith, K. M. and Williams, R. C. Endeavour 17: 12-21, 1958. Younghusband, H. B. and Lee, P. E. Virology 38: 247-254,1969. Manyakov, V. F. J. gen. Virol. 36: 73-79,1977. Williams, R. C. and Smith, K. M. Nature 179: 119-120,1957. Caspar, D. L. D. and Klug, A Cold Spring Harbor Symp. Quant. BioI. 27: 1-24, 1957. Matthews, R. E. F. Intervirology 17: 56-58,1982. Willis, D. , Goorha, R. and Chinchar, V.

0 at room temperature (RT) or 4°C for 20-30 min. After centrifugation at 10,000 rpm for 20 min (or 13,000 rpm for 15 min in a micro-centrifuge), the tubes were inverted for 1-3 h and excess PEG was wiped from the sides of the tube with a sterile cotton swab. The phage pellets were resuspended in 600 ~l STE and extracted once with an equal volume of phenol, twice with phenol: chloroform: isoamyl alcohol (25:24:1) and once with chloroform: isoamyl alcohol (24:1) before ethanol precipitation. 1 mM EDTA).

And Lee, P. E. Virology 51: 409-423, 1973. Horwitz, M. S. Virology (Ed. B. N. Fields). Raven Press, New York. p. 433-476, 1985. Roizman, B. and Batterson, W. Virology (Ed. B. N. Fields). Raven Press, New York. p. 497-526, 1985. , Lee, P. E. and Seligy, V. L. Isolation and characterization of the Tipu/a iridescent virus capsid gene.

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