Mycotoxin Protocols (Methods in Molecular Biology Vol 157) by Mary W. Trucksess, Albert E. Pohland

By Mary W. Trucksess, Albert E. Pohland

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For analyses using a parallel ELSD, one would omit scrubbing distilled water with the organic sieve. The sieve often contaminates the water (and sample blanks) with resins which, although they contain no UV-chromophore and hence are not observed by UV detection, would nevertheless give a strong background signal in an ELSD. 2. In this specific example, we made aqueous solutions of fumonisin B1 at about 1 mg/mL. This high FB1 concentration allowed the detection of minor components present at levels as low as a few µg/mL.

9. Solutions are stable for over one year. 4. Notes 1. The standard solution preparation requires utmost care to avoid calculation, measuring, and dilution errors and contamination or use of the wrong solvents. 2. Weigh using an analytical balance, or for smaller quantities, a microbalance. Aflatoxins must be handled in a glove box, because of their carcinogenicity and their tendency to disperse in the environment as a result of their electrostatic properties. 3. This dissolution may take several hours and require the use of a mechanical shaker for some toxins; an example is aflatoxin B2 in toluene-acetonitrile (9 + 1).

And Cox, R. H. (1981) Handbook of Toxic Fungal Metabolites. Academic Press, New York. 3. , Trucksess, M. , and Page, S. W. 22): Collaborative Study. J. AOAC Int. 82, 251–258. Electrospray Mass Spectrometry 37 5 Electrospray Mass Spectrometry for Mycotoxin Detection and Purity Analysis Jon G. Wilkes and Jackson O. Lay, Jr. 1. Introduction Gas Liquid Chromatography (GLC) has been used to separate a few mycotoxin types: trichothecenes, zearalenone, patulin, and anthraquinones (1–9). In contrast, most of the known mycotoxins are amenable to high performance liquid chromatographic (HPLC) separation (10).

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