Normal Human Tissue and Cell Culture B. Endocrine, by Curtis C. Harris, Benjamin F. Trump and Gary D. Stoner

By Curtis C. Harris, Benjamin F. Trump and Gary D. Stoner (Eds.)

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Extra info for Normal Human Tissue and Cell Culture B. Endocrine, Urogenital, and Gastrointestinal Systems

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18 DAVID G. KAUFMAN et al. 1. HUMAN ENDOMETRIUM IN ORGAN CULTURE 19 During the simulated “secretory phase” when the medium contained both progesterone and estrogen, incorporation of [3H]Thdresulted in a lower DNA specific activity. Incorporation of radioactive precursors in organ cultures was also evaluated by autoradiography. Again, the extent of incorporation of radioactive precursors appeared comparable to that observed in the freshly obtained tissue. Autoradiographic grains were localized to cell nuclei when [3H]Thdwas incorporated, but overlay both nuclei and cytoplasm in cultures in which [3H]Urd or [3H]Leu had been incorporated.

21. Autoradiogramof [3H]leucine labeling in human endometrial tissue in the middle of the secretory phase (day 21) of the menstrual cycle. At this point in the menstrual cycle there is a considerably higher grain density. Giemsa, X 1200. 22. ” Labeled cells are located primarily in the epithelium of the glands and the grains are localized over cell nuclei. Giemsa, X 1OOO. FIG. 23. ” Although not evident in this field, stromal cells are among those which are labeled under these culture conditions.

43, XL-XLI. This Page Intentionally Left Blank METHODS IN CELL BIOLOGY, VOLUME 21B Chapter 2 Tissue Culture of the H u m a n Uterine Cervix GEORGE D. WILBANKS, EDMUND LEIPUS, AND DAVID TSURUMOTO Department of Obstetrics and Gynecology, Rurb Medical College, Rnsb Presbyterian St. Luker Medical Center, Cbicago, Illinois I. Introduction . . . . . . . . . . . A. S c o p e . . . . . . . . . . . B. Review of Methodology and Results . . . . II. Materials and Methods . . . .

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