Pilze: Eine unterhaltsame Einführung in die Mykologie by Hans W. Kothe, Erika Kothe

By Hans W. Kothe, Erika Kothe

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Integration Is Key Biosensor technologists strive for the simplest possible solution to measurement in complex matrices. While notable success has been achieved with indi- Enzyme Biosensors 35 vidual sensors, pragmatic solutions to many problems involve the construction of a sensor system in which the carefully optimized performance of the sensor is supported by associated electronics, fluidics, and separation technology. In process monitoring, for example, the process must remain inviolate while the sensor frequently requires protection from the process and its products.

Breyer, R. , Hu, J. , Knight, K. , Lim, W. , et al. (1991) Random mutagenesis of protein sequences using oligonucleotide cassettes. Methods Enzymol. 208, 564–586. 88. Bornscheuer, U. , and Meyer, H. H. (1998) Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epithilones. Biotechnol. Bioeng. 58, 554–559. 89. , and Momose, H. (1998) Engineering of a cold-adapted protease by sequential random mutagenesis and a screening system. Appl. Environ.

In addition, although the volume appears very small, the droplet size of 50–100 µm is relatively large compared to the size of the transducer structures that can be produced. Other liquid-handling techniques include automated syringe-type processes, the best known of which is often referred to as Cavro deposition and usually involves “touching off” a droplet onto a surface. Another method involves picking up reagents on a “pin” that possesses a concave head and depositing it onto the surface of the device, a technique adapted from pharmaceutical applications.

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