Plant Protoplasts and Genetic Engineering V by Y. H. Dan, C. T. Stephens (auth.), Professor Dr. Y. P. S.

By Y. H. Dan, C. T. Stephens (auth.), Professor Dr. Y. P. S. Bajaj (eds.)

In continuation of Volumes eight, nine, 22, and 23, this new quantity offers with the regeneration of crops from remoted protoplasts and genetic transformation in quite a few species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus.
These stories replicate the far-reaching implications of protoplast expertise in genetic engineering of vegetation. The publication encompasses a wealth of necessary info for complex scholars, academics, and researchers within the box of plant tissue tradition, molecular biology, genetic engineering, plant breeding, and basic biotechnology.

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After fusion, washing, and transfer of the cells to liquid medium, it eould be determined that approx. 5% of the cells present were heterofusion products. We are now able to fuse successfully mesophyll protoplasts, suspension protoplasts and cytoplasts in various combinations from a range of sugar beet and fodder beet lines using PEG. 1 Physiology As part of oUf work on asymmetrie somatie hybridization, we investigated the bioehemieal and physiologieal impact of radiation on protoplasts as a means of parent (nucleus) inactivation.

Planta 171: 235-240 Gilmour DM, Davey MR, Cocking EC (1989) Production of somatic hybrid tissues following chemical and e1ectrical fusion of protoplasts from albino cell suspensions of M edicago sativa and M. borealis. Plant Cell Rep 8: 29-32 Günther G, Baumann I, Bogs R, Knösche R (1986) Comparative cytological investigations on protoplasts, tissue cultures and seedlings from Beta vulgaris (sugar-beet). In: Int. Symp Nuclear techniques and in vitro culture for plant improvement. IAEA/FAO, Vienna, pp 83-89 Gupta P, Ganesh R, Mohanty P (1982) Effect ofsome senescence retardant pretreatments on yield of protoplasts from spinach beet (Beta vulgaris) leaves.

The high plating efficiency, colony formation, and plant regeneration achieved in this investigation offer an opportunity to introduce genes of Fusariurn-resistant A. densifiorus Sprengeri or Myersii into asparagus cv. Lucullus 234 through protoplast fusion or genetic transformation. 22-/lm filter for 16 to 17 h in the dark at 27 to 28°C on a gyratary shaker at 30 rpm. 85 (CPW wash solution), and pelleted in each ca se by centrifugation at 70 g for 5 min. 85) on top of which 3 ml of epw wash solution was layered after centrifugation at 150 g for 5 min.

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